Rakesh Rathore, Ph.D.
Genome Research Institute
University of Cincinnati
2180 E. Galbraith Rd., 131 GRI-B
Cincinnati, OH 45237-0505
Ph: 513-558-4057
Fx: 513-558-5061



Email Rakesh


Related Links

• MS based HTS
• Research News_UC health News
• Interview on SAB
• Personal Webpage
• SBS
• ABRF

Honors
Publications

Cancer Hospital & Research Institute, MS-Applied Biochemistry
G.B. Pant University of Agri. & Technology, PhD-Biochemistry (minor in Molecular Biology)

ÖREBRO UNIVERSITY & GÖTEBERG UNIVERSITY, Sweden,Postdoctoral- Bacterial Molecular Biology & Membrane protein Biochemistry
ALBANY MEDICAL CENTER, Research Associate/Postdoctoral - Calcium Signaling and Smooth muscle cells

I specialized in Biochemistry & Molecular biology during my studies at Cancer Hospital & Research Institute and G. B. Pant University. I then joined the Membrane protein Biochemistry group at Gothenburg University/Orebro University, Sweden. After two year, I joined Cardiovascular Sciences Department at Albany Medical Center, NY in a lab focusing on the Calcium Signaling particularly regulation and functions of calcium influx and calcium release in Vascular and Airway smooth muscles. My research work involved advanced Biochemical (Radioactive, Fluorescence & Chemiluminescence based assay development), Molecular Biology, Cell Biology, Microbiological & Microscopy techniques (Confocal Microscopy) and Animal model studies.

My current research at University of Cincinnati is focused toward Assay development and integration of MS based High-throughput screening technologies. We are working in close collaboration with MDS analytical technologies, Canada.


Honors:
- 2009 - present, reviewer for the Journal of Biomolecular Screening.

- Thermo Scientific ABRF Outstanding Scientist/Technologist Award,2009.

- Honorable Mentions award in Bioscience Technology's 2009 Researcher of the Year.

- ABRF 2009, outstanding poster award.

- Carl Trygger Foundation, Sweden (Postdoctoral scholarship), 2001-2003.

- CSIR, Senior Research Fellowship, 1997-2000.

- Travel grants awarded by DST and NAAS for 6th IPMB Congress, Quebec, Canada,2000.

- Travel grants awarded by the Genetics Trust of India for 3rd ICS Congress, Hamburg, Germany,2000.

- First prize for the paper/poster presented at DRDO Conference,1998.


Presentations:

• Rakesh Rathore, Jay J. Corr, and Kenneth D Greis. Balancing the MRM transition of small molecules, substrate/product conversion for enhances label-free enzyme inhibitor screening. Presented at the 57th ASMS Conference on Mass Spectrometry & Allied Topics, Philadelphia, Pennsylvania, May 31-June 4 2009

• Rakesh Rathore, Jay J. Corr, and Kenneth D. Greis. Multiplexing Enzyme assays for Inhibition Screening via MALDI-3Q-MS. The Society for Biomolecular Sciences 15th Annual Conference & Exhibition, Lille, France April 26-30, 2009.

• Pauline J. Vollmerhaus, Rakesh Rathore, Jay J. Corr,and Kenneth D. Greis. MALDI triple quadrupole mass spectrometry: A high throughput acquisition tool in enzyme inhibitor screening. The 237th ACS National Meeting, Salt Lake City, UT, March 22-26, 2009.

• Rakesh Rathore , Jay J. Corr, and Kenneth D. Greis. Extending MALDI-QqQ-MS Enzyme Screening Assays to Targets with Small Molecule Substrates. The 13th annual international meeting of the ABRF on Application and Optimization of Existing and Emerging Biotechnologies, Memphis, Tennessee. February 7-10, 2009.

• Rakesh Rathore, Wendy D. Haffey, Kelly Rask, Hongjun Shu, Michael Wyder, and Kenneth D. Greis. Proteomics, Biological Mass Spectrometry, and High Throughput Inhibitor Screening at the University of Cincinnati. The 13th annual international meeting of the ABRF on Application and Optimization of Existing and Emerging Biotechnologies, Memphis, Tennessee. February 7-10, 2009.

• Kenneth D Greis, Rakesh Rathore, , George Scott, Pauline Vollmerhaus and Jay J.Corr. Multiplex enzyme inhibition screening via MALDI-3Q-MS .Presented at the 56th ASMS Conference on Mass Spectrometry & Allied Topics,Denver,Colorado, June 2008.

• Rakesh Rathore, Yun-Min Zheng, and Yong-Xiao Wang.Title: Hypoxia activates NADPH oxidase through mitochondrial ROS–PKCepsilon signaling axis in pulmonary artery smooth muscle cells, 51 th Annual Meeting of Biophysical Society, Baltimore, Maryland, March 3-7, 2007.

• Qing-Hua Liu, Rakesh Rathore, Yun-Min Zheng, and Yong-Xiao Wang. Title: Role of Intracellular Calcium Release and Sensitivity in Airway Hyper-responsiveness, 51 th Annual Meeting of Biophysical Society, Baltimore, Maryland, March 3-7, 2007.

• Ling Dong, Yun-Min Zheng, Rakesh Rathore, Dee Van Riper, Qing-Hua Liu, and Yong-Xiao Wang. Title: Functional and molecular evidence for the impaired activity of calcium-activated potassium channels in diabetic cerebral artery smooth muscle cells, 51 th Annual Meeting of Biophysical Society, Baltimore, Maryland, March 3-7, 2007.

• Rakesh Rathore, Yun-Min Zheng, Harold A Singer & Yong-Xiao Wang. Title: Acute hypoxia activates PKCepsilon in pulmonary arteries through mitochondria-derived reactive oxygen species, 50 th Annual Meeting of Biophysical Society, Salt lake city, Utah, Feb 18-22, 2006.

• Yun-Min Zheng, Rakesh Rathore, Qing song Wang, Harold A. Singer, Vincenzo Sorrentino, Michael I. Kotlikoff. 2004. Title: Functional Role of Ryanodine receptors in Pulmonary Artery Smooth Muscle Cells, 48 th Annual Meeting of Biophysical Society, Baltimore, Maryland, Feb 14-18,2004.

• Rakesh Rathore, Helena Sävenstrand, Pal Nyrén and Åke Strid .2001.Fusion of the membrane- bound proton-translocating pyrophospatase with GFP for facilitation of purification and crystallization. In: poster presentation in International course`` Molecular approaches to study gene expression and protein function`` at Dept of Bioscience, Karolinska Institute, Stockholm, Sweden, Sep 15-19, 2001.

• 3rd International Crop Science Congress, Hamburg, Germany, August 17-22, 2000. Poster title: Development of insect resistance in pigeonpea [Cajanus cajan (L.) Millsp.] using tomato protease inhibitor (TPI-II) gene.

•6th International Congress of Plant Molecular Biology, Quebec, Canada, June 18-24, 2000. Poster title: Genetic transformation studies in pigeonpea [Cajanus cajan (L.) Millsp.].




Publications

1:

Rathore R, Corr JJ, Lebre DT, Seibel WL, Greis KD Extending matrix-assisted laser desorption/ionization triple quadrupole mass spectrometry enzyme screening assays to targets with small molecule substrates. Rapid Commun Mass Spectrom. 2009 Sep;23(20):3293-3300. PMID: 19757451 [PubMed - indexed for MEDLINE]

2:	Liu QH, Zheng YM, Korde AS, Yadav VR, Rathore R, Wess J, Wang YX Membrane depolarization causes a direct activation of G protein-coupled receptors leading to local Ca2+ release in smooth muscle. Proc Natl Acad Sci U S A. 2009 Jun; PMID: 19549818 [PubMed - indexed for MEDLINE]

3:	Rathore R, Corr J, Scott G, Vollmerhaus P, Greis KD Development of an Inhibitor Screening Platform via Mass Spectrometry. J Biomol Screen. 2008 Nov; PMID: 19029013 [PubMed - indexed for MEDLINE]

4:	Li XQ, Zheng YM, Rathore R, Ma J, Takeshima H, Wang YX Genetic evidence for functional role of ryanodine receptor 1 in pulmonary artery smooth muscle cells. Pflugers Arch. 2008 Jul; PMID: 18663468 [PubMed - indexed for MEDLINE]

5:

Rathore R, Zheng YM, Niu CF, Liu QH, Korde A, Ho YS, Wang YX Hypoxia activates NADPH oxidase to increase [ROS]i and [Ca2+]i through the mitochondrial ROS-PKCepsilon signaling axis in pulmonary artery smooth muscle cells. Free Radic Biol Med. 2008 Nov;45(9):1223-31. PMID: 18638544 [PubMed - indexed for MEDLINE]

6:	Zheng YM, Wang QS, Liu QH, Rathore R, Yadav V, Wang YX Heterogeneous gene expression and functional activity of ryanodine receptors in resistance and conduit pulmonary as well as mesenteric artery smooth muscle cells. J Vasc Res. 2008 ;45(6):469-79. PMID: 18434746 [PubMed - indexed for MEDLINE]

7:

Dong L, Zheng YM, Van Riper D, Rathore R, Liu QH, Singer HA, Wang YX Functional and molecular evidence for impairment of calcium-activated potassium channels in type-1 diabetic cerebral artery smooth muscle cells. J Cereb Blood Flow Metab. 2008 Feb;28(2):377-86. PMID: 17684520 [PubMed - indexed for MEDLINE]

8:

Rathore R, Zheng YM, Li XQ, Wang QS, Liu QH, Ginnan R, Singer HA, Ho YS, Wang YX Mitochondrial ROS-PKCepsilon signaling axis is uniquely involved in hypoxic increase in [Ca2+]i in pulmonary artery smooth muscle cells. Biochem Biophys Res Commun. 2006 Dec;351(3):784-90. PMID: 17087917 [PubMed - indexed for MEDLINE]

9:

Zheng YM, Wang QS, Rathore R, Zhang WH, Mazurkiewicz JE, Sorrentino V, Singer HA, Kotlikoff MI, Wang YX Type-3 ryanodine receptors mediate hypoxia-, but not neurotransmitter-induced calcium release and contraction in pulmonary artery smooth muscle cells. J Gen Physiol. 2005 Apr;125(4):427-40. PMID: 15795312 [PubMed - indexed for MEDLINE]

10:	Rathore RS, Chand L Plantlet regeneration from decapitated embryonic axes of pigeonpea [Cajanus cajan (L.) Millsp.]. Indian J Exp Biol. 1999 May;37(5):496-8. PMID: 19691174 [PubMed - indexed for MEDLINE]