FURTHER INFORMATION
Kenneth D. Greis, Ph.D.
Associate Professor
Director, Proteomics & Mass Spectrometry
Department of Cancer and Cell Biology
2180 E. Galbraith Road, Rm B109
Cincinnati, OH 45237
513 558-7102
513 558-5061 Fax

Email Ken


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Funding
Publications
Ken Greis, PhD


Centre College, B.S.--Biology

University of Kentucky, Ph.D.--Biochemistry

Johns Hopkins Medical School, Postdoctoral--Glycobiology/Virology

Univ. of Alabama at Birmingham, Postdoctoral--Glycobiology/Mass Spectrometry


My research focuses on technology development and the application of mass spectrometry to understand biochemical and biomedical systems. This direction was initiated in the early 1990s while studying glycosylation and phosphorylation in herpes viruses and has continued to this day in areas of protein characterization, proteomics and compound screening. Between 1996-2006, I built and lead protein characterization and proteomics mass spectrometry groups within two pharmaceutical companies. In those roles, I developed significant expertise in mass spectrometry and proteomics while collaborating in a variety of research areas including: signal transduction, angiogenesis, metabolic regulation, anti-infectives, musculoskeletal, inflammation and cancer. In July 2006, I joined the University of Cincinnati, as an Associate Professor in the Department of Genome Sciences (now Cancer and Cell Biology). In this role, I am responsible for developing and applying advanced proteomics technologies both as core resources and as a collaborator with a variety of invesitgators. I also lead a centralized effort across the University of Cincinnati College Medicine and the Cincinnati Children’s Hospital Medical Center in the role as Director of Proteomics. Finally, my research interests beyond proteomics and protein characterization include the development of a novel mass spectrometry-based compound screening approach as a label-free alternative to traditional high throughput screening assays. For additional information on Proteomics Laboratory services or the Department, click on the links at the top left of this page.


Funding Support
UC Millennium Scholar Fund
Cincinnati Children's Research Foundation
US Department of Defense
MDS Analytical Technologies
NIDDK, NIAID, NIEHS, NCI


Publications
1: Aslan JE, You H, Williamson DM, Endig J, Youker RT, Thomas L, Shu H, Du Y, Milewski RL, Brush MH, Possemato A, Sprott K, Fu H, Greis KD, Runckel DN, Vogel A, Thomas G
Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with TRAIL-induced apoptosis.
Mol Cell. 2009 May;34(4):497-509.
PMID: 19481529 [PubMed - indexed for MEDLINE]
2: Suzuki M, Wiers K, Brooks EB, Greis KD, Haines K, Klein-Gitelman MS, Olson J, Onel K, Oʼneil KM, Silverman ED, Tucker L, Ying J, Devarajan P, Brunner HI
Initial Validation of a Novel Protein Biomarker Panel for Active Pediatric Lupus Nephritis.
Pediatr Res. 2009 Feb;
PMID: 19218887 [PubMed - indexed for MEDLINE]
3: Haffey WD, Mikhaylova O, Meller J, Yi Y, Greis KD, Czyzyk-Krzeska MF
iTRAQ proteomic identification of pVHL-dependent and -independent targets of Egln1 prolyl hydroxylase knockdown in renal carcinoma cells.
Adv Enzyme Regul. 2008 Dec;
PMID: 19159641 [PubMed - indexed for MEDLINE]
4: Rathore R, Corr J, Scott G, Vollmerhaus P, Greis KD
Development of an Inhibitor Screening Platform via Mass Spectrometry.
J Biomol Screen. 2008 Nov;
PMID: 19029013 [PubMed - indexed for MEDLINE]
5: Eismann T, Huber N, Shin T, Kuboki S, Galloway E, Wyder M, Edwards MJ, Greis KD, Shertzer HG, Fisher AB, Lentsch AB
Peroxiredoxin-6 Protects Against Mitochondrial Dysfunction and Liver Injury During Ischemia/Reperfusion in Mice.
Am J Physiol Gastrointest Liver Physiol. 2008 Nov;
PMID: 19033532 [PubMed - indexed for MEDLINE]
6: Mikhaylova O, Ignacak ML, Barankiewicz TJ, Harbaugh SV, Yi Y, Maxwell PH, Schneider M, Van Geyte K, Carmeliet P, Revelo MP, Wyder M, Greis KD, Meller J, Czyzyk-Krzeska MF
The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress.
Mol Cell Biol. 2008 Apr;28(8):2701-17.
PMID: 18285459 [PubMed - indexed for MEDLINE]
7: Greis KD
Mass spectrometry for enzyme assays and inhibitor screening: an emerging application in pharmaceutical research.
Mass Spectrom Rev. ;26(3):324-39.
PMID: 17405133 [PubMed - indexed for MEDLINE]
8: Greis KD, Zhou S, Burt TM, Carr AN, Dolan E, Easwaran V, Evdokimov A, Kawamoto R, Roesgen J, Davis GF
MALDI-TOF MS as a label-free approach to rapid inhibitor screening.
J Am Soc Mass Spectrom. 2006 Jun;17(6):815-22.
PMID: 16616859 [PubMed - indexed for MEDLINE]
9: Greis KD, Zhou S, Siehnel R, Klanke C, Curnow A, Howard J, Layh-Schmitt G
Development and validation of a whole-cell inhibition assay for bacterial methionine aminopeptidase by surface-enhanced laser desorption ionization-time of flight mass spectrometry.
Antimicrob Agents Chemother. 2005 Aug;49(8):3428-34.
PMID: 16048957 [PubMed - indexed for MEDLINE]
10: Thompson LJ, Wang F, Proia AD, Peters KG, Jarrold B, Greis KD
Proteome analysis of the rat cornea during angiogenesis.
Proteomics. 2003 Nov;3(11):2258-66.
PMID: 14595824 [PubMed - indexed for MEDLINE]
11: Peters KG, Davis MG, Howard BW, Pokross M, Rastogi V, Diven C, Greis KD, Eby-Wilkens E, Maier M, Evdokimov A, Soper S, Genbauffe F
Mechanism of insulin sensitization by BMOV (bis maltolato oxo vanadium); unliganded vanadium (VO4) as the active component.
J Inorg Biochem. 2003 Aug;96(2-3):321-30.
PMID: 12888267 [PubMed - indexed for MEDLINE]
12: Delinsky DC, Greis KD
Capillary chromatography-coupled mass spectrometry with column switching for rapid identification of proteins from 2-dimensional electrophoresis gels.
J Proteome Res. ;1(3):279-84.
PMID: 12645905 [PubMed - indexed for MEDLINE]
13: Isfort RJ, Wang F, Greis KD, Sun Y, Keough TW, Bodine SC, Anderson NL
Proteomic analysis of rat soleus and tibialis anterior muscle following immobilization.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Apr;769(2):323-32.
PMID: 11996498 [PubMed - indexed for MEDLINE]
14: Isfort RJ, Wang F, Greis KD, Sun Y, Keough TW, Farrar RP, Bodine SC, Anderson NL
Proteomic analysis of rat soleus muscle undergoing hindlimb suspension-induced atrophy and reweighting hypertrophy.
Proteomics. 2002 May;2(5):543-50.
PMID: 11987128 [PubMed - indexed for MEDLINE]
15: Egnaczyk GF, Greis KD, Stimson ER, Maggio JE
Photoaffinity cross-linking of Alzheimer's disease amyloid fibrils reveals interstrand contact regions between assembled beta-amyloid peptide subunits.
Biochemistry. 2001 Oct;40(39):11706-14.
PMID: 11570871 [PubMed - indexed for MEDLINE]
16: Smaill JB, Rewcastle GW, Loo JA, Greis KD, Chan OH, Reyner EL, Lipka E, Showalter HD, Vincent PW, Elliott WL, Denny WA
Tyrosine kinase inhibitors. 17. Irreversible inhibitors of the epidermal growth factor receptor: 4-(phenylamino)quinazoline- and 4-(phenylamino)pyrido[3,2-d]pyrimidine-6-acrylamides bearing additional solubilizing functions.
J Med Chem. 2000 Apr;43(7):1380-97.
PMID: 10753475 [PubMed - indexed for MEDLINE]
17: Isfort RJ, Hinkle RT, Jones MB, Wang F, Greis KD, Sun Y, Keough TW, Anderson NL, Sheldon RJ
Proteomic analysis of the atrophying rat soleus muscle following denervation.
Electrophoresis. 2000 Jun;21(11):2228-34.
PMID: 10892733 [PubMed - indexed for MEDLINE]
18: Sanchez V, Greis KD, Sztul E, Britt WJ
Accumulation of virion tegument and envelope proteins in a stable cytoplasmic compartment during human cytomegalovirus replication: characterization of a potential site of virus assembly.
J Virol. 2000 Jan;74(2):975-86.
PMID: 10623760 [PubMed - indexed for MEDLINE]
19: VanBogelen RA, Greis KD, Blumenthal RM, Tani TH, Matthews RG
Mapping regulatory networks in microbial cells.
Trends Microbiol. 1999 Aug;7(8):320-8.
PMID: 10431205 [PubMed - indexed for MEDLINE]
20: Fry DW, Bridges AJ, Denny WA, Doherty A, Greis KD, Hicks JL, Hook KE, Keller PR, Leopold WR, Loo JA, McNamara DJ, Nelson JM, Sherwood V, Smaill JB, Trumpp-Kallmeyer S, Dobrusin EM
Specific, irreversible inactivation of the epidermal growth factor receptor and erbB2, by a new class of tyrosine kinase inhibitor.
Proc Natl Acad Sci U S A. 1998 Sep;95(20):12022-7.
PMID: 9751783 [PubMed - indexed for MEDLINE]
21: Stevenson TI, Loo JA, Greis KD
Coupling capillary high-performance liquid chromatography to matrix-assisted laser desorption/ionization mass spectrometry and N-terminal sequencing of peptides via automated microblotting onto membrane substrates.
Anal Biochem. 1998 Sep;262(2):99-109.
PMID: 9750124 [PubMed - indexed for MEDLINE]
22: Greis KD, Hart GW
Analytical methods for the study of O-GlcNAc glycoproteins and glycopeptides.
Methods Mol Biol. 1998 ;76():19-33.
PMID: 9664344 [PubMed - indexed for MEDLINE]
23: Greis KD, Zhu S, Matalon S
Identification of nitration sites on surfactant protein A by tandem electrospray mass spectrometry.
Arch Biochem Biophys. 1996 Nov;335(2):396-402.
PMID: 8914938 [PubMed - indexed for MEDLINE]
24: Greis KD, Hayes BK, Comer FI, Kirk M, Barnes S, Lowary TL, Hart GW
Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by beta-elimination and tandem electrospray mass spectrometry.
Anal Biochem. 1996 Feb;234(1):38-49.
PMID: 8742080 [PubMed - indexed for MEDLINE]
25: Hayes BK, Greis KD, Hart GW
Specific isolation of O-linked N-acetylglucosamine glycopeptides from complex mixtures.
Anal Biochem. 1995 Jun;228(1):115-22.
PMID: 8572267 [PubMed - indexed for MEDLINE]
26: Hart GW, Greis KD, Dong LY, Blomberg MA, Chou TY, Jiang MS, Roquemore EP, Snow DM, Kreppel LK, Cole RN
O-linked N-acetylglucosamine: the "yin-yang" of Ser/Thr phosphorylation? Nuclear and cytoplasmic glycosylation.
Adv Exp Med Biol. 1995 ;376():115-23.
PMID: 8597237 [PubMed - indexed for MEDLINE]
27: Greis KD, Gibson W, Hart GW
Site-specific glycosylation of the human cytomegalovirus tegument basic phosphoprotein (UL32) at serine 921 and serine 952.
J Virol. 1994 Dec;68(12):8339-49.
PMID: 7966627 [PubMed - indexed for MEDLINE]
28: Thomas JR, McConville MJ, Thomas-Oates JE, Homans SW, Ferguson MA, Gorin PA, Greis KD, Turco SJ
Refined structure of the lipophosphoglycan of Leishmania donovani.
J Biol Chem. 1992 Apr;267(10):6829-33.
PMID: 1551889 [PubMed - indexed for MEDLINE]
29: Greis KD, Turco SJ, Thomas JR, McConville MJ, Homans SW, Ferguson MA
Purification and characterization of an extracellular phosphoglycan from Leishmania donovani.
J Biol Chem. 1992 Mar;267(9):5876-81.
PMID: 1556103 [PubMed - indexed for MEDLINE]
30: Crawford IT, Greis KD, Parks L, Streips UN
Facile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki.
J Bacteriol. 1987 Dec;169(12):5423-8.
PMID: 3680166 [PubMed - indexed for MEDLINE]